Abstract
Purpose of Review
At present, the most common diagnostic measures for the autoimmune neuromuscular disease myasthenia gravis (MG) are radioimmunoprecipitation assay (RIPA), enzyme-linked immunosorbent assay (ELISA), and cell-based assay (CBA). Considering the pitfalls of these diagnostic assays, this review describes the advantages of using Xenopus tissue cultures for MG diagnosis and research.
Recent Findings
Our recent study described a novel CBA involving Xenopus tissue cultures for MG serological diagnosis. Moreover, this CBA can potentially be applied to elucidate the pathogenic mechanisms underlying acetylcholine receptor endocytosis and degradation and to develop and validate potential therapeutic strategies for MG.
Summary
Although most CBAs are relatively labor intensive, Xenopus CBA is a promising tool for the initial clinical serological diagnosis and for the pathological research of MG. The future studies will be devoted to gain a better understanding of the etiology of MG and to provide a therapeutic intervention for this disease.
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2jGfpqR
via IFTTT
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου