Τρίτη 31 Ιανουαρίου 2017

Metabonomics by proton nuclear magnetic resonance in human pleural effusions: A route to discriminate between benign and malignant pleural effusions and to target small molecules as potential cancer biomarkers

BACKGROUND

Cytopathology is a noninvasive and cost-effective method for detecting cancer cells in pleural effusions (PEs), although in many cases, the diagnostic performance is hindered by the paucity of significant cells or the lack of clear morphological criteria. This study presents the results of an omics approach to improving the diagnostic performance of PE cytology.

METHODS

Metabolic profiling with proton nuclear magnetic resonance (1H-NMR) was performed for 92 PEs (44 malignant cases of 8 different cancers and 48 benign cases of 7 nonneoplastic conditions). Light's criteria were used to further classify PEs as transudates or exudates, and 1H-NMR spectroscopy was used to differentiate malignant pleural effusions (mPEs) from benign pleural effusions (bPEs).

RESULTS

1H-NMR metabolic analysis showed clearly different spectra for mPEs and bPEs in the regions of the signals due to lipids, branched amino acids, and lactate, which were increased in mPEs. Transudates and exudates in bPEs were differentiated as well on the basis of the 1H-NMR signals from lipids and lipoproteins, which were increased in exudates.

CONCLUSIONS

Subject to validation in further larger studies, 1H-NMR metabonomics could be an effective and reliable ancillary tool for PE investigations and diagnoses. Cancer (Cancer Cytopathol) 2017. © 2017 American Cancer Society.



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