Seminal plasma caspase 3, cytochrome c and total antioxidant capacity in oligospermic males and association with sperm indices

2016-12-12T08-16-11Z
Source: Journal of Experimental and Integrative Medicine
Abiodun Mathias Emokpae, Happy Ngozi Chima, Muhammed Ahmed.
Objective: To determine the levels of cytochrome c, caspase 3 and total antioxidant capacity (TAC) in oligospermic male subjects in Zaria, northern Nigeria. Materials and methods:The study participants were 56 oligospermic male subjects attending fertility clinic and 30 control subjects who were males of proven fertility with normal semen parameters. Semen samples were collected by masturbation after a minimum of 3 days sexual abstinence into sterile containers, were allowed to liquefy at room temperature and manual semen analysis was performed according to WHO standard. Seminal plasma cytochrome c, caspase 3 and total antioxidant capacity were assayed by sandwiched ELISA technique using reagents supplied by Elabscience and Wkea Medical supplies corporation, China respectively. The differences in the mean levels of measured parameters were compared using Students t test and caspase 3 was correlated with sperm indices. Results: The mean values of cytochrome C and caspase 3 were significantly higher (p


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