Transcriptome analysis of phycocyanin inhibitory effects on SKOV-3 cell proliferation

Publication date: 1 July 2016
Source:Gene, Volume 585, Issue 1
Author(s): Jun Ying, Jian Wang, Huijuan Ji, Chaoqing Lin, Ruowang Pan, Li Zhou, Yulong Song, Enyong Zhang, Ping Ren, Jishun Chen, Qian Liu, Teng Xu, Huiguang Yi, Jinsong Li, Qiyu Bao, Yunliang Hu, Peizhen Li
Phycocyanin (PC) from Spirulina platensis has inhibitory effects on tumor cell growth. In this research, the transcriptome study was designed to investigate the underlying molecular mechanisms of PC inhibition on human ovarian cancer cell SKOV-3 proliferation. The PC IC50 was 216.6μM and 163.8μM for 24h and 48h exposure, respectively, as determined by CCK-8 assay. The morphological changes of SKOV-3 cells after PC exposure were recorded using HE staining. Cells arrested in G2/M stages as determined by flow cytometry. The transcriptome analysis showed that 2031 genes (with > three-fold differences) were differentially expressed between the untreated and the PC-treated cells, including 1065 up-regulated and 966 down-regulated genes. Gene ontology and KEGG pathway analysis identified 18 classical pathways that were remarkably enriched, such as neurotrophin signaling pathway, VEGF signaling pathway and P53 signaling pathway. qPCR results further showed that PTPN12, S100A2, RPL26, and LAMA3 increased while HNRNPA1P10 decreased in PC-treated cells. Molecules and genes in those pathways may be potential targets to develop treatments for ovarian cancer.



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