The proliferation of chronic lymphocytic leukemia (CLL) cells requires communication with the lymphoid organ microenvironment. Integrin-linked kinase (ILK) is a multifunctional intracellular adaptor protein that transmits extracellular signals to regulate malignant cell motility, metastasis, and cell cycle progression, but is poorly characterized in hematological malignancies. In this study, we investigated the role of ILK in the context of CLL and observed high ILK expression in patient samples, particularly in tumor cells harboring prognostic high risk markers such as unmutated IGHV genes, high Zap70 or CD38 expression, or a signature of recent proliferation. We also found increased numbers of Ki67 (MKI67)-positive cells in regions of enhanced ILK expression in lymph nodes (LNs) from CLL patients. Using co-culture conditions mimicking the proliferative LN microenvironment, we detected a parallel induction of ILK and cyclin D1 (CCND1) expression in CLL cells that was dependent on the activation of NF-κB signaling by soluble TNFα. The newly synthesized ILK protein co-localized to centrosomal structures and was required for correct centrosome clustering and mitotic spindle organization. Furthermore, we established a mouse model of CLL in which B cell-specific genetic ablation of ILK resulted in decelerated leukemia development due to reduced organ infiltration and proliferation of CLL cells. Collectively, our findings describe a TNFα-NF-κB-mediated mechanism by which ILK expression is induced in the LN microenvironment, and propose that ILK promotes leukemogenesis by enabling CLL cells to cope with centrosomal defects acquired during malignant transformation.-
from #AlexandrosSfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/1SfqZ7P
via IFTTT
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
Abstract Epilepsy is a serious neurological disorder which disturbs the common activities of a human brain. It is detected with the help o...
-
The main idea behind this work was demonstrated in a form of a new thermoelectrochromic sensor on a flexible substrate using graphene as an ...
-
Tetracycline Repressors (TetRs) modulate multi-drug efflux pathways in several pathogenic bacteria. In Streptomyces, they additionally regul...
-
Abstract There are many unanswered questions about giant cell tumor (GCT) treatment and not enough attention is paid to the biomechanics o...
-
Bearing faults are the biggest single source of motor failures. Artificial Neural Networks (ANNs) and other decision support systems are wid...
-
Nowadays, sound classification applications are becoming more common in the Wireless Acoustic Sensor Networks (WASN) scope. However, these a...
-
The paper proposes a multicriteria decision analysis (MCDA) framework for a comparative evaluation of nuclear waste management strategies ta...
-
Tuberculosis (TB) is one of the leading causes of death worldwide, especially in developing countries. Neonatal BCG vaccination occurs in va...
-
Pathological angiogenesis in the eye is an important feature in the pathophysiology of many vision-threatening diseases, including retinopat...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου