Σάββατο 2 Ιανουαρίου 2021

Paediatric Dermatology

Genetics of Vascular Malformations: Current Perspectives
Kin Fon Leong

Indian Journal of Paediatric Dermatology 2021 22(1):1-11

For decades, vascular anomalies are categorized as either vascular tumors or malformations based on their onset, clinical course, radiologic, and histologic features. Owing to the heterogeneity of vascular anomalies, they are frequently misdiagnosed. With the advent of massively parallel next-generation sequencing, the molecular landscape of vascular anomalies is rapidly evolving and recent discoveries have shed light on the genetic basis and classification of these vascular disorders. The genotype-phenotype correlation will provide a more precise classification of vascular anomalies and form the basis for future targeted pharmacologic therapy. Thus far, inhibitor of mTOR, AKT1, and PIK3CA (sirolimus, miransertib, and alpelisib) have promising clinical results. In vascular malformations, majority of sporadic cases are due to somatic mutations that disrupt the main endothelial receptor intracellular signaling pathways, i.e., PIK3CA-AKT-mTOR, RAS - MAPK – ERK, and SMAD signaling pathways. Most of the sporadic vascular malformations are caused by somatic mutations that are acquired after fertilization, instead of being inherited from his parents. In general, this type of mosaicism is not inherited, except when the mutation affects the gonads.


Cosmetic Procedures in Adolescents: What's Safe and What Can Wait


Indian Journal of Paediatric Dermatology 2021 22(1):12-20

Teenagers between 13 and 19 years are increasingly seeking cosmetic procedures. They are suffering from anxiety, depression, and low self-esteem as a result of an obsession with body image and celebrity culture, fueled by social networking sites. Teenagers seek cosmetic procedures most commonly for traumatic scars, acne and acne scars, pigmentary abnormalities, hypertrichosis, hirsutism, and tattoo removal. They demand plastic surgery for nose deformities, breast asymmetry, ear abnormalities, and congenital deformities. The physical, emotional, psychological, social, ethical, and legal aspects must be considered while counseling adolescents. Not every teenager seeking cosmetic surgery is well suited for a procedure, and teens must demonstrate emotional maturity and an understanding of the limitations of these procedures and the risks involved. There should be a 3-month cooling-off period, followed by another consultation, which should be done in the presence of a parent. Only very essential surgery should be performed, giving realistic expectations on the outcome of procedures, as they rely too much on physical appearance to gain confidence. A psychiatric evaluation is essential to rule out body dysmorphic disorders in those repeatedly seeking treatment for minor defects. Sometimes, procedures are necessary to avoid social withdrawal and loss of self-esteem. Proper informed consent should be taken, explaining the benefits, limitations, and risks involved. Ideally, teenagers should not receive cosmetic or surgical procedures unless there are compelling medical or psychological reasons to do so. A successful aesthetic procedure in a mature teenager can have a positive influence, whereas surgery on an immature, psychologically unstable adolescent can have an adverse impact. This review discusses what is safe and what can wait, still there is limited evidence. There is a strong need for guidelines for the use of cosmetic surgery on children and teenagers.


Role of Elimination Diet in Atopic Dermatitis: Current Evidence and Understanding


Indian Journal of Paediatric Dermatology 2021 22(1):21-28

Atopic dermatitis (AD) is a fairly common dermatosis of childhood. In many cases, parents are concerned that food allergy plays a role in their child's symptoms and they ask whether practicing elimination diets would help in controlling the symptoms. In this review, we try to provide concise answers to the questions raised by the parents, apart from addressing the myths associated with elimination diets in AD and provide scientific evidence in favor of or against the common notions. A comprehensive English-language literature search for the role of elimination diet in AD across multiple databases (PubMed, EMBASE, MEDLINE, and Cochrane) for keywords (alone and in combination) was performed. MeSH as well as non-MeSH terms such as “AD,” “diet elimination,” “treatment,” “prevention,” “evidence,” “blanket elimination,” and “specific exclusion diet” were taken into consideration. There is level I evidence to support specific exclusion diets in preselected and screened patients, but there is insufficient evidence to recommend strict (blanket) elimination diets.


Pattern of Dermatoses in Small Children in a Tertiary Care Hospital


Indian Journal of Paediatric Dermatology 2021 22(1):29-36

Aims: The aim of the study was to study the pattern of dermatoses in children up to 5 years of age. Settings and Design: It is an observational study done in a tertiary care hospital. Subjects and Methods: A total of 505 patients, aged up to 5 years, attending the department of dermatology, venereology, and leprosy for a duration of 18 months from January 2017 to June 2018 were included in the study. Statistical Analysis Used: All results were statistically analyzed by IBM SPSS software and Chi-square test was used to compare the associations. Results: Preschoolchildren (75.44%) formed the majority. Males (54.65%) outnumbered females. Infections constituted the most (45.94%) followed by eczema (21.98%), hypersensitivity disorders (14.25%), nevi (4.55%), papulosquamous (3.76%), pigmentary (3.76%), physiological and transient noninfective neonatal conditions (3.56%), sweat gland (2.97%), hair disorders (0.59%), and other dermatoses (2.97%). Conclusions: Infections/infestations constituted the most among the dermatoses in children up to 5 years of age. As they are preventable, educating the parents, guardian, care takers, and teachers in the preschool about communicable diseases can limit their transmission and improve the well-being of children.


Clinical Spectrum of Cutaneous Adverse Drug Reactions in Pediatric Population in East Coast of Andhra Pradesh: An Observational Study
VV V Satyanarayana, Suruthi Purushothaman, Bonu Chandipriya

Indian Journal of Paediatric Dermatology 2021 22(1):37-42

Context: Cutaneous adverse drug reaction (CADR) among pediatric population is underappreciated and often misdiagnosed in clinical settings. Furthermore, epidemiologic data on only cutaneous adverse reactions among this population are limited. Aim: The aim of this study is to analyze the CADRs with reference to the pattern, causative drugs, its subgroup analysis, and other clinical characteristics among children. Settings and Design: This was an observational, hospital-based study over a period of 12 months. Materials and Methods: The CADRs occurring and referred to the inpatient and outpatient department of dermatology were actively monitored and the collected reports were analyzed for CADR pattern, drug groups, clinicodemographic profile, and severity of adverse drug reaction (ADR). Statistical Analysis Used: Data were documented in Microsoft Excel and analyzed using SPSS Version 20.0. Results: A total of 33 CADRs were documented. Most of the ADRs (40%) occurred between the age of 11 and 15 years. Antimicrobials comprised the major group of drugs causing ADRs (42.4%). Maculopapular rash (54.5%) and urticaria (15.2%) were the most common type of ADR. A single case of death due to anticonvulsant-induced toxic epidermal necrolysis had been observed in the study period. There were more occurrences of ADRs with multiple drugs compared to single-drug therapy. About 80% of reactions were of mild to moderate. Conclusion: Knowledge of the pattern and the offending drug helps in better diagnosis, management, reduced complications in this population, and also helps in preventing recurrences, thereby helps in promoting the safe use of drugs, which can overall impact the quality of health care among children positively.


Acne Fulminans: A Case Report and Review of Literature


Indian Journal of Paediatric Dermatology 2021 22(1):43-47

Acne fulminans (AF), a variant of acne vulgaris, is a rare disease that occurs after treatment of AF with oral isotretinoin. Less than 200 cases of AF have been reported in literature, mostly in young men and all with varying clinical presentations. A thorough search of literature was performed for AF using PubMed, MEDLINE, EMBASE, and UK PubMed Central electronic databases. Pathogenesis is not clearly established. However, it is most likely due to hypersensitivity reactions to sebum or bacterial antigens. It presents as sudden eruption of friable, hemorrhagic crusting overlying the ulcers, plaques, and tender nodules, usually on the trunk, with or without systemic symptoms such as malaise, fever, and arthralgia a few days after initiation of oral isotretinoin therapy. Laboratory abnormalities including raised erythrocyte sedimentation rate, altered hematological profile, and radiological evidence of osteolytic bone lesions may be found. Treatment involves the use of oral steroids with subsequent addition of oral isotretinoin. Many other immunosuppressive therapies have been tried.


Clinical and Dermoscopic Spectrum: Novel Findings in Interesting Cases


Indian Journal of Paediatric Dermatology 2021 22(1):48-51

Uncommon, interesting clinical entities presenting in dermatological practice often challenges the diagnostic skills of a trained dermatologist. A non-invasive tool for recognition of these conditions is especially relevant in the paediatric population and dermoscopy plays a pivotal role in diagnosing these cases. A spectrum of novel clinical and dermoscopic findings is reported in four interesting cases. (vitiligo ponctue, cutaneous larva migrans, zosteriform angiokeratoma, linear milia en plaque).


Norwegian (Crusted) Scabies Involving Eyelids and Conjunctiva


Indian Journal of Paediatric Dermatology 2021 22(1):52-55

Scabies is a parasitosis caused by the mite sarcoptes scabiei var. hominis, with crusted scabies being more contagious than classic scabies because of a larger mite burden. It can lead to an epidemic of classic scabies if not diagnosed early and treated accordingly. We report a 10-year-old boy who presented with a complaint of an inability to open his left eye and irritation in the right eye. Examination showed the presence of multiple crusted lesions throughout the body, including face and eyelids, and a yellowish patch in the right bulbar conjunctiva. The histopathological evaluation of the specimen of the conjunctival lesion confirmed it to be sarcoptes infestation. Face and eyelids involvement are very rarely in crusted scabies and only a few cases have been reported in the past. Our report demonstrates conjunctiva as an unusual site of involvement in these cases.


Localized Pseudoxanthomatous Mastocytosis of Vulva


Indian Journal of Paediatric Dermatology 2021 22(1):56-58

Cutaneous mastocytosis (CM) is a mast cell proliferative disorder that is common in the pediatric age group. Common entities are urticaria pigmentosa, solitary mastocytoma, telangiectasia macularis eruptive perstans. and diffuse CM. Pseudoxanthomatous mastocytosis presents in the form of yellowish nodular lesion resembling xanthoma and is a rare variant and not reported much in the literature. We, hereby report a rare case of localized vulvar pseudoxanthomatous mastocytosis in a 10-year-old female child.


Curious Subcutaneous Swellings: A Case of Pediatric Myocysticercosis


Indian Journal of Paediatric Dermatology 2021 22(1):59-61

Cysticercosis is a parasitic infection caused by the larval stage of the tape worm, Cysticercus cellulosae, chiefly affecting the subcutaneous tissue, central nervous system, orbit, and the skeletal muscles. It occurs in human due to ingestion of contaminated water or raw meat of pig. We hereby present a case of a 6-year-old female presenting with multiple subcutaneous swellings of 3 months' duration, associated with painful movement and associated with a history of on and off fever and malaise. Local part ultrasonography revealed multiple cystic lesions in the muscular plane, with peripheral streak of calcification indicative of scolex. Fine-needle aspiration cytology of the lesions showed revealed degenerated and viable polymorphs, macrophages, few lymphocytes, and foreign body giant cells with foci of necrosis, with occasional hooklets of parasite, with findings suggestive of parasitic infection in neck with acute on chronic inflammation. Computed tomography (CT) scan of brain was normal. Thus, clinical, radiological, and cytological findings confirmed the diagnosis of myocysticercosis. This case is presented because of the rareness of its occurrence.



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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
Telephone consultation 11855 int 1193,

Dermatology

High prevalence of high-risk cutaneous squamous cell carcinoma in the thrace region of Turkey
Sezgi Sarikaya Solak, Haydar Yöndem, Yildiz Gursel Urun, Mert Cezik, Nuray Can

Turkish Journal of Dermatology 2020 14(4):83-89

Background: The characteristics of cutaneous squamous cell carcinoma (cSCC) may show differences according to the geographic distribution and ethnicity. Although most cSCCs are treated with surgical excision or other local interventions, high-risk cSCCs may have poor outcome. In the present study, we aimed to evaluate the clinicopathological characteristics and determine the high-risk features of cSCCs in the Thrace region of Turkey where the information on cSCCs is scarce. Methods: We retrospectively investigated the biopsy-proven cSCCs diagnosed between the years 2014 and 2018, in a tertiary university hospital and evaluated the high-risk features. Results: A total of 211 cSCCs were included. Men with cSCC were significantly younger than women with cSCC (P <.001). Almost one-half of the tumors (n = 103) were located on the lower lip. Patients with a cSCC on the lower lip were younger than those with a cSCC on the other sites. All patients with a cSCC on the ears were men. Twenty-eight percent of patients had tumor size more than 20 mm and 28% had tumor thickness more than 6 mm. Immunosuppression was present in 7.6% of patients. A total of 177 (83.9%) patients had high-risk cSCC. The local recurrence rate was 6.2%, and the metastasis rate was 5.2%. Conclusions: The Thrace region has significant number of cSCC, and high-risk features are very frequent. Clinicians should carefully evaluate the cSCCs in terms of high-risk features.


CYP4F22 gene mutations in patients with autosomal recessive congenital ichthyosis: Identification of two novel mutations
Esra Arslan Ates, Hüseyin Onay, Ilgen Ertam, Esra Ataman, Filiz Hazan, Asude Durmaz, Tugrul Dereli, Ferda Özkinay

Turkish Journal of Dermatology 2020 14(4):90-94

Background: Autosomal recessive congenital ichthyosis (ARCI) is a genetically heterogeneous keratinization disorder, which is clinically classified into five main forms: Lamellar ichthyosis, congenital ichthyosiform erythroderma, harlequin ichthyosis, self-healing collodion baby, and bathing suit ichthyosis. Mutations in TGM1, ABCA12, ALOX12B, ALOXE3, NIPAL4, CYP4F22, PNPLA1, LIPN, and CERS3 genes have been described in patients with ARCI. However, in 20% of the ARCI patients, the genetic defect remains unknown. Materials and Methods: In this study, we investigated the mutations in the CYP4F22 gene in ARCI patients who do not have mutations in two common ARCI genes, NIPAL4 and TGM1. Twenty-two patients diagnosed with ARCI and having no mutations in TGM1 and NIPAL4 genes were included in the study. Their CYP4F22 genes were sequenced using the Sanger sequencing method. Results: In 5 of 22 (22.7%) ARCI patients, four different mutations, of which two were previously reported, were found. The two novel mutations were c.976C> T and c.1189C> T. The c.727C> T and c.1303C>T mutations were previously reported. Conclusions: This study expands the CYP4F22 mutation spectrum and to provide more accurate genetic counseling for patients at risk.


Androphenotypic features in patients with coronary artery disease
Gülsüm Gençoglan, Fatmagül Gülbaşaran, Işıl Inanir, Uğur Kemal Tezcan, Kamer Gündüz

Turkish Journal of Dermatology 2020 14(4):95-98

Objective: It has been a debate whether phenotypic features are associated with increased risk of coronary heart disease. Proposed explanations for this relation include biological aging, individual susceptibilities, and androgens which contribute to both the atherosclerotic process and dermatological signs. The results of the studies are inconsistent and most are not based on cardiovascular imaging techniques. Here, association between androphenotypic features and the risk and severity of coronary artery disease (CAD) in men is evaluated. Methods: This case–control study consists of 166 male patients with angiography-proven CAD and 160 age-gender-matched controls. Gensini score of angiograms (for severity of CAD) and phenotypic characteristics including androgenetic alopecia (AGA), thoracic hairiness (TH), hair graying a diagonal earlobe crease (DEC), and hairy ear (HE) were recorded. Men with well-established cardiovascular risk factors were excluded. Results: AGA, DEC, and HE were significantly more frequent in patients with CAD than controls (98.2% and 83.1% [P < 0.001], 61.4% and 23.8% [P < 0.001], 69.3% and 50.6% [P = 0.001], respectively). As the severity of AGA increased, the incidence of heart disease was increasing in patients. The presence of TH and AGA was found to be related to higher Gensini scores. Conclusion: The exact mechanism between these phenotypic features and CAD still remains to be elucidated. However, observation of visible aging signs is easy and inexpensive. AGA, HE, and DEC may be used as early screening tools for CAD.


Misleading clinical presentation of a palmar lichen nitidus masquerading as pompholyx
Ghazal Ahmed, Satyaki Ganguly, Hitesh Yadav

Turkish Journal of Dermatology 2020 14(4):99-101

Lichen nitidus is usually a chronic localized disease of unknown etiology having multiple differentials. A 10-year-old boy presented with itchy, multiple, discrete, grouped, and minute papules with surrounding erythema in some and exfoliation in few others, involving the center of the right palm, the palmar aspect of the left little finger with few lesions over the dorsal surface of both the hands. The presenting feature misguided us with a few clinical points which are unusual in lichen nitidus. We report the case to highlight the clinical mimicry and limitations of clinical assessment for diagnosing lichen nitidus.


Psoriasis vulgaris developing in healed pemphigus vulgaris: A rare case of epitope spread or isotopic response?
Ravindranath Brahmadeo Chavan, Vasudha A Belgaumkar, Nitika Sanjay Deshmukh, Ranjitha Krishnegowda

Turkish Journal of Dermatology 2020 14(4):102-105

Although psoriasis and autoimmune blistering diseases are considered to be disorders with completely different etiopathogenesis, literature has documented a few cases of psoriasis associated with bullous diseases, particularly bullous pemphigoid. Here, we report the case of a 30-year-old male presenting with multiple flaccid blisters and erosions, clinically and histopathologically consistent with the diagnosis of pemphigus vulgaris. Although all these lesions resolved after two doses of dexamethasone cyclophosphamide pulse therapy, he returned 3 weeks later with multiple erythematous scaly plaques developing over the postinflammatory areas, compatible with the diagnosis of psoriasis vulgaris, which necessitated a modification in the treatment protocol. This rare case highlights the diagnostic and therapeutic challenges accompanying this unique scenario and attempts to elucidate the probable pathogenic mechanisms underlying the co-existence (simultaneous or sequential) of these two apparently unrelated dermatoses.


Phacomatosis pigmentokeratotica associated with unilateral toe walking due to short achilles tendon
Andac Salman, Ayse Deniz Yucelten, Ozlem Akin Cakici, Olcay Unver

Turkish Journal of Dermatology 2020 14(4):106-108

Phacomatosis pigmentokeratotica (PPK) is characterized by the co-occurrence of speckled lentiginous nevus (nevus spilus) and an organoid nevus with or without extracutaneous involvement. The extracutaneous manifestations may vary widely with musculoskeletal, neurologic, ocular, and vascular findings. The PPK is also associated with an increased risk of cutaneous or extracutaneous tumors. Therefore, the patients with PPK should be followed up regularly for possible malignant transformation. Here, we report a 5-year-old boy with PPK associated with toe walking due to short Achilles tendon, which was not previously reported, to our knowledge.



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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
Telephone consultation 11855 int 1193,

Pulmonology

Chronic obstructive pulmonary disease: A review about gender differences
Carolina Santos, Tiago Pereira, Raquel Barros

Eurasian Journal of Pulmonology 2020 22(3):135-143

The major risk factor for chronic obstructive pulmonary disease (COPD) is smoking. COPD is thought to be traditionally a male illness, but its prevalence in women is increasing because they are adopting lifestyle habits similar to men. A literature review of publications indexed in MEDLINE, Latindex, SciELO, and DOAJ databases was carried out. Were considered 44 articles with relevance to the topic addressed. The literature review aim was to characterize the gender susceptibility differences in COPD development as well as the changes that this disease may induce in the lung function. Through the present literature review, it was verified that there are multiple aspects that contribute to gender inequalities in COPD development. Among them are genetic predisposition, hormonal factors, tobacco smoke components metabolization, anatomical and physiological characteristics, bronchial hyperreactivity, and noxious agent's exposure. Gender differences in deleterious effects of tobacco smoke on lung function do not hold consensus, as there are authors reporting a greater lung functional decline in women even when less exposed to harmful substances, while others have found no differences in many of lung functional parameters. The studies analyzed were different regarding methodology and sample characteristics, which may contribute to results discrepancy obtained by the researchers. COPD affects men and women in increasingly similar proportions, so it is important to identify and characterize the particularities of tobacco smoke effects in both genders to improve the knowledge about the disease.


How are radiological, spirometric and quality of life measures related to each other in cases of bronchiectasis
Burcu Arpinar Yigitbas, Celal Satici, Elif Yelda Niksarlıoğlu

Eurasian Journal of Pulmonology 2020 22(3):144-152

Background: Radiological, spirometric, and quality-of-life (QoL) measures are essential parameters influencing the prognosis of patients with bronchiectasis (BE). However, to date, few studies have evaluated these measures together. OBJECTIVE: The study objective is to assess the relationships between high resolution computed tomography, spirometric and QoL scores considered in the steady and exacerbation states of BE. METHODS: We reviewed retrospectively patients who had been diagnosed with BE. Ninety-two cases were deemed eligible and completed the Short Form-36 (SF-36) and St George's Respiratory Questionnaire (SGRQ). A statistical assessment looking for correlations between HCRT, spirometry and QoL questionnaires was performed. Besides, factors for the modified Bhalla score (MBS) and clinical exacerbations were evaluated. RESULTS: İn the exacerbation state, patients' spirometric parameters and the domains of the SF-36 were even more strongly correlated with MBS, in particular, symptom duration, exacerbation and hospitalization rates in the previous year. Linear regression models for the steady and exacerbation state revealed SF-36 domains, forced expiratory volume in 1 s predicted and symptom duration were more related to MBS. In addition, the exacerbation rate was related to the domains of the SF-36, MBS and hospitalization within the previous year in both the steady and exacerbation state of BE. CONCLUSIONS: As a result of this study, SF-36, which is rarely used in clinical practice, has been demonstrated to be more correlated with radiological and pulmonary function test (PFT) scores than SGRQ. Assessing the patient's disease status can be performed more efficiently if MBS and SF-36 are combined with PFT.


Evaluation of paraoxonase-1 enzyme activity and oxidative stress relations in malignant mesothelioma cases
Didem Turgut Cosan, Güntülü Ak, Emine Çolak, Aylin Dal, Çağrı Öner, Ahu Soyocak, Ertuğrul Çolak, Hasan Veysi Güneş, Muzaffer Metintaş

Eurasian Journal of Pulmonology 2020 22(3):153-157

BACKGROUND: Malignant pleural mesothelioma (MPM) is the most common cancer in the pleura and highly aggressive with a very poor prognosis. Asbestos, known as a carcinogenic mineral with fiber structures, is the main cause of MPM formation. Exposure to asbestos causes an increase in reactive oxygen species, deficiency of antioxidant enzyme levels, and DNA damage. As a result of asbestos pathogenesis, all of these changes cause pulmonary fibrosis, pleural diseases, and malignancies. The endogenous antioxidant paraoxonase-1 (PON-1) is a calcium-dependent esterase involved in the hydrolysis of lipid peroxides, and PON-1 has been shown to have protective properties in oxidative stress and inflammatory diseases in various studies. OBJECTIVE: The study aimed to examine the relationship of MPM with PON-1 enzyme activity and oxidative status using total oxidant status (TOS) and total antioxidant status (TAS). MATERIALS AND METHODS: The study population was formed of 33 retrospectively examined mesothelioma patients as MPM group and 33 age- and sex-matched healthy individuals as controls. PON-1 activity was measured spectrophotometrically by enzyme-linked immunosorbent assay method. Total antioxidant and oxidant status was determined using Rel Assay Diagnostics kit. Oxidative stress index (OSI) was estimated as the ratio of the TOS to the TAS levels. RESULTS: In the present study, PON-1, TOS, TAS, and OSI levels were adjusted by comorbidity and smoking. The results indicated that TOS and OSI of MPM patients increased compared to healthy controls (P < 0.001 for both). The results also demonstrated the decrease of PON-1 activity and TAS in MPM cases (P < 0.001, for both). CONCLUSION: These results suggested that oxidative stress occurring as a result of inhalation of asbestos fibers may reduce the level of PON-1.


Antifibrotic treatment in patients with idiopathic pulmonary fibrosis: Our experience in 41 cases
Berna Akıncı Ozyurek, Derya Yenibertiz, Aslıhan Gürün Kaya, Sertaç Büyükyaylacı Özden, Yurdanur Erdoğan

Eurasian Journal of Pulmonology 2020 22(3):158-162

INTRODUCTION: It has been shown that antifibrotic agents (pirfenidone and nintedanib), used in the treatment of idiopathic pulmonary fibrosis (IPF) in recent years, decelerate the worsening of pulmonary function tests and the progression of the disease and also reduce the frequency of acute exacerbations and hospitalizations. In this study, we aimed to evaluate the results of antifibrotic treatment that we have been using since 2013 in our clinic. MATERIALS AND METHODS: Forty-one patients diagnosed as IPF between August 1, 2013, and February 1, 2019, in the eighth clinic of our hospital were included in this study. The information of the patients was obtained from the patient files. Data were analyzed by descriptive statistical methods, Kolmogorov–Smirnov test, and Wilcoxon test. RESULTS: Thirty-eight patients were male and three patients were female. The mean age was 65.6 ± 7.0 years. The diagnosis of 34 patients was made clinically and radiologically, and 7 patients were diagnosed pathologically. The longest usage time of antifibrotic drugs was 5.5 years in 2 patients, and the minimum usage time was 6 months in 2 patients. Thirty-four patients were using pirfenidone and seven patients were using nintedanib according to the data of their last visit. There was no significant difference between the baseline 6-min walk test results and the 6th-month, 1st-year, 2nd-year, 3rd-year, and 4th-year results. A significant decrease was determined in diffusing capacity of the lungs for carbon monoxide (DLCO) test results of the 6th month and 1st year compared to baseline (baseline: 63%, 6th month: 57%, and 1st year: 43%) (P < 0.05). There was no significant difference in DLCO test results of the 2nd, 3rd, and 4th year compared to baseline (P > 0.05). A significant decrease was determined in forced vital capacity (FVC) results of the 2nd year compared to baseline (68% and 59%, respectively) (P < 0.05). There was no significant difference in the FVC results of the 6th month, 1st year, 3rd year, and 4th year compared to baseline (P > 0.05). CONCLUSION: Similar to the literature, we have experienced that antifibrotic drugs decelerate the progression of the disease, reduce the risk of developing exacerbations, and are more tolerable in terms of side effect profile compared to the previous treatments.


Evaluation of the sleep quality of patients admitted to the internal medicine outpatient clinic
İdris Kirhan, Fatih Üzer

Eurasian Journal of Pulmonology 2020 22(3):163-168

AIM: This study aimed to determine the sleep quality and the factors affecting sleep quality in patients admitted to the internal medicine outpatient clinic. MATERIALS AND METHODS: The study was conducted between July 1, 2019, and July 15, 2019, with a face-to-face questionnaire administered to the patients who applied to the internal medicine outpatient clinic of Harran University. A descriptive questionnaire, Pittsburgh Sleep Quality Index (PSQI), and Epworth Sleepiness Scale (ESS) were used in the study. RESULTS: One-hundred and twelve (48.0%) male and 121 (51.9%) female patients with a mean age of 38.5 ± 16.3 years (18–86 years) were included in the study. The total PSQI mean was 4.9 ± 3.0 and the score range was 0–15. The rate of those with poor sleep quality was 48.9%. The mean score of ESS was 5.0 ± 3.6, and 12 (30%) patients admitted to the outpatient clinic experienced excessive sleepiness during the day. Gender of the participants (P = 0.800), income level (P = 0.113), the size of the house they were living in (P = 0.783), body mass index (BMI) (P = 0.491), and comorbid diseases (P = 0.803) did not affect the sleep quality; the number of people living in the same house affected the sleep quality (P < 0.001). It was found that those with a high BMI (≥30) had significantly worse sleep quality than those without. Sleep quality decreased as the number of people living at home decreased. CONCLUSION: We found that almost half of the patients admitted to the internal medicine outpatient clinic had poor sleep quality. We also found that as the number of people living in the same home decreases, sleep quality deteriorates.


Bronchodilator reversibility: What are the differences between asthma and chronic obstructive pulmonary disease?
Raquel Barros, Patrícia Araújo, Cláudia Mourato, Khrytyna Budzac, Ana Sofia Oliveira, Cristina Bárbara

Eurasian Journal of Pulmonology 2020 22(3):169-174

INTRODUCTION: Currently, the bronchodilator reversibility is not recommended to differentiate asthma from chronic obstructive pulmonary disease (COPD); however, physiopathological specificities of each disease contribute to the differences in response to the drug. OBJECTIVES: The objective of this study is to evaluate the differences in bronchodilator response between asthmatic and COPD patients and to determine which of the bronchodilation criteria have the best ability to detect the positive response in these patients. MATERIALS AND METHODS: This was a cross-sectional study. The sample included 104 patients with asthma or COPD who performed lung function tests between January and March 2018. The whole sample was analyzed according to postbronchodilator variation (Δ) of lung function parameters, and the postbronchodilator reversibility was characterized using a multiple bronchodilation criteria. The drug used in reversibility test was salbutamol. RESULTS: In this study, Δ forced-expiratory volume in the 1st s (ΔFEV1) and a Δ Raw was statistically higher in the group with asthma compared with the group with COPD. In the asthma group, the criteria ↓ functional residual capacity (FRC) ≥10%, ↓Raw ≥ 35%, ↑ forced expiratory flow between 25% and 75% of vital capacity (FEF25%–75%) ≥20% and ↑ FEV1 and / or ↑ forced vital capacity ≥12% and 200 mL were those that presented a greater capacity of detecting a positive response to bronchodilator. The criteria ↑ FEF25%–75%≥20% and ↓ FRC ≥ 10% were those that had the greater ability of detecting airway reversibility in COPD group. CONCLUSION: The analysis of postbronchodilator FEV1 and raw modifications as well as the using of a combination of multiple bronchodilation criteria contribute to a deeper characterization of bronchodilator reversibility in asthma and COPD.


Subclinical peripheral neuropathy in patients with chronic obstructive pulmonary disease without hypoxemia
Hatice Yurtgun, Baykal Tülek, Hakan Ekmekçi, Fikret Kanat, Mecit Süerdem

Eurasian Journal of Pulmonology 2020 22(3):175-179

AIM: The aim of the study was to determine the prevalence of subclinical peripheral neuropathy (PNP) in stable chronic obstructive pulmonary disease (COPD) patients without severe hypoxemia. MATERIALS AND METHODS: Fifty-six (52 men and 4 women) patients with COPD without severe hypoxemia, 25 healthy smokers, and 24 healthy nonsmokers were included in the study. The latency, amplitude, and velocity measurements of right and left median motor nerve, tibial motor nerve, peroneal motor nerve, median sensory nerve, sural sensory nerve, right ulnar motor nerve, and right ulnar sensory nerve were performed. RESULTS: A high proportion of PNP was detected in the COPD group compared to the smoker and nonsmoker control groups (41.1%, 36.0%, and 33.3%, respectively). However, the difference between the groups was not statistically significant (P = 0.784). However, some of the electrophysiological measurements were statistically significantly worse in the COPD group (P < 0.05). In the COPD group, a correlation was not detected between PNP and duration of COPD, age, body mass index, smoking status (pack/year), forced vital capacity %, forced expiratory volume in 1 s %, SO2, and C-reactive protein values (P > 0.05). CONCLUSION: The present study demonstrates that the PNP may be an extrapulmonary manifestation of COPD. The physician should be aware of the possibility of PNP in COPD patients without severe hypoxemia.


Isolated pulmonary artery vasculitis
Meltem Agca, Bedrettin Yildizeli, Emine Bozkurtlar, Simge Yavuz, Tulin Sevim

Eurasian Journal of Pulmonology 2020 22(3):180-183

We present a unique case of a 26-year-old woman, which first evaluated as sarcoma or thromboembolic events with their clinical and radiological similarities of the pulmonary artery but diagnosed as isolated pulmonary artery vasculitis after endarterectomy with the help of histopathological examinations. After the endarterectomy procedure, the other causes that may lead to vasculitis had been excluded. The patient had the immunosuppressive therapy after the procedure, and her control computerized tomography revealed that the lumen of the pulmonary artery was wide open. Isolated pulmonary artery vasculitis must be considered as a differential diagnosis when a filling defect of the pulmonary artery had been detected without other systemic findings.


A case of diffuse endobronchial metastasis of rectum carcinoma presenting with symptoms of diffuse airway obstruction and respiratory failure
Serap Argun Baris, Ece Sahinoglu, Ilknur Basyigit

Eurasian Journal of Pulmonology 2020 22(3):184-186

Endobronchial metastases of extra pulmonary tumors are rare. This report presents a case with diffuse endobronchial metastases with rectum carcinoma. A 63-year-old non-smoker female was admitted to our outpatient clinic with dyspnea and dry cough for two months. She had a history of rectum carcinoma and resection surgery eight months ago. On physical examination, wheezing was remarkable. There was patchy consolidation in both lung fields on chest x-ray. PET-CT revealed widespread nodules in pulmonary parenchyma which were consistent with rectal cancer metastasis. Bronchoscopy was performed and revealed extensive mucosal infiltration and multiple various sizes of polypoid lesions in the distal trachea and both main lobar bronchus. The histopathological evaluation reported as metastasis of rectum carcinoma. The oncological medical treatment regimen and radiotherapy was also planned. She admitted to emergency department with chest pain and progressive dyspnea 2 months after the diagnosis. Thorax CT demonstrated that increased metastatic peribronchial infiltrates, septal thickening and endobronchial polypoid lesions which was consisted with lymphangitic carsinomatosis and endobronchial metastasis. There was hypercapnic respiratory failure according to arterial blood gases analysis. She was intubated and admitted to the intensive care unit (ICU) and died in ICU on 7th day of admission. This case indicates that the possibility of endobronchial metastasis should be considered in a patient with underlying malignancy. If available bronchoscopic intervention should be planned not to let misdiagnosis.


Acute inhalation injury after marijuana use: A hidden cause
Selen Karaoglanoglu, Emine Serap Yilmaz, Irem Karaman, Sevket Ozkaya

Eurasian Journal of Pulmonology 2020 22(3):187-190

Marijuana is one of the most extensively smoking substance all around the world, although not common in our society. Its effects on lung are similar to tobacco, causing increased cough, sputum, hyperinflation, and rapid bronchodilatation following with possible airway obstruction. Chronic usage of marijuana may cause a variety of conditions such as bronchitis, large bullae formation, and pneumonia. Here, we report an uncommon case of hidden marijuana smoking causing acute inhalation injury. A 39-year-old male had consulted clinics with sudden onset of dyspnea, cough, and nausea. His chest X-ray and thorax computed tomography have revealed peripheral opacities with ground-glass infiltrations. After a detailed review of history, he was diagnosed with acute inhalation injury due to hidden marijuana smoking. As a conclusion, clinicians who encounter with individuals who have concurrent inhalation damage need to take a careful exposure history and should be alert for the possibilities of further complications and a worsening clinical picture.



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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
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Παρασκευή 1 Ιανουαρίου 2021

The tubarial glands



The tubarial glands: Discovered but not defined – A narrative review
Tarun Kumar Suvvari, Nithya Arigapudi

Journal of Radiation and Cancer Research 2020 11(4):140-141

A pair of salivary glands, named as tubarial glands, was found between the nasal cavity and throat, i.e., at the nasopharynx's lateral walls, overlaying the torus tubarius by the Netherlands Cancer Institute while working on radiation toxicity among prostate cancer patients. The tubarial glands were identified using prostate-specific membrane antigen imaging using positron emission tomography coupled with computed tomography, which is used to detect the spread of prostate cancer. The anatomy, physiology, oncological study of the glands, and data's interpretation and limitations from the research to date have been discussed.

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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
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Nervous System Diseases

Comparison of factor structure and psychometric properties of original and abbreviated version of the Penn State Worry Questionnaire in a nonclinical sample: a cross-sectional psychometric study
Alireza Rashtbari, Hossein Malekizadeh, Omid Saed

Asia Pacific Journal of Clinical Trials: Nervous System Diseases 2020 5(4):43-50

Background and objectives: Worry as a main symptom of generalized anxiety disorder is a chain of repetitive and uncontrollable thoughts about possible negative events in the future. The Penn State Worry Questionnaire is one of the most widely used measures for assessing pathological worry. The purpose of the present study was to investigate the psychometric properties of the abbreviated version of the Penn State Worry Questionnaire-Abbreviated (PSWQ-A) and to compare it with the original version of the Penn State Worry Questionnaire (PSWQ) in a nonclinical sample. Participants and methods: The present study is a cross-sectional psychometric study. The statistical population of this study consisted of all students studying at Zanjan University of Medical Sciences (n = 3500) from January to October 2018. A sample of 350 people was selected for the study. Research measures were the PSWQ-A, PSWQ, and the Generalized Anxiety Disorder 7-item (GAD-7) Scale. The present study was carried out after approval of Social Determinants of Health Research Center of Zanjan University of Medical Science with the project code of A-12-924-5 on October 7, 2017. The project was also approved by the Ethics Committee of Zanjan University of Medical Sciences on October 17, 2017 and the approval ID was IR.ZUMS.REC.1396.187. Results: Exploratory factor analysis, scree plot, and parallel analysis supported the single factor structure PSWQ-A. The total variance explained by the single-factor model of PSWQ-A was higher (53.1% versus 49.1%). Generally, fit indices for the PSWQ-A was better fitted than the PSWQ. Both measures had acceptable convergent validity (r=0.52 for both questionnaires) and satisfactory internal consistency (α=0.87 for both questionnaires). Conclusion: PSWQ-A has better psychometric properties compared to PSWQ, and it can be used for faster and more accurate assessment of worry in psychological studies and therapeutic settings.


Key enzymes of glutamate metabolisms in the brain of neonatal and adult rats exposed to monosodium glutamate
Uche Stephen Akataobi

Asia Pacific Journal of Clinical Trials: Nervous System Diseases 2020 5(4):51-57

Background and objectives: Despite the effective role of monosodium glutamate as a food additive, there are claims indicating that monosodium glutamate consumption increases the level of glutamate an excitatory neurotransmitter which can be toxic to the brain in accumulated level. The present study attempted to understand the differential effect of monosodium glutamate on key enzymes of glutamate metabolisms in rat brain exposed either as neonate or adult to monosodium glutamate. Methods: The rat neonates were divided into six groups with seven animals per group and exposed to different concentrations of monosodium glutamate as neonates only (normal saline or monosodium glutamate 4 mg/g), neonate plus adults (monosodium glutamate 5 or 10 mg/g) and adult only (monosodium glutamate 5 or 10 mg/g). Key enzymes of glutamate metabolisms were measured in whole brain homogenates. All experiments were approved by the Faculty of Basic Medical Sciences University of Calabar and ethics committee-04/11/2018. Results: Except neonate plus adult 5 mg/g group, glutamate dehydrogenase and glutamate synthetase activities were significantly higher in administered groups than in the control group (P < 0.05). There was no significant difference in glutamate synthetase activity among monosodium glutamate administered groups (P > 0.05). The glutamate carboxylase activity was significantly higher in all monosodium glutamate administered groups than in the control group (P < 0.05). The brain alanine aminotransferase and aspartate aminotransferase activities of rats in each monosodium glutamate administered group increased in a dose-dependent manner (P < 0.05). Conclusion: Exposure to monosodium glutamate can increase the activities of key enzymes of glutamate metabolism in the brain of neonate and adult rats similarly, which is not determined by age difference.


Effect of Ayurveda gut therapy protocol in managing dysbiosis of children with autism: study protocol for a randomized controlled trial
Dinesh Karayil Subramanian, Anita Patel, Madathaniyil Joseph George, Swapna Chitra Sugunanandagopan, Santhi Krishna, Sujitha Variyattukunnu Kelu, Jayakrishnan Kalluvirath, Archana Madhavi

Asia Pacific Journal of Clinical Trials: Nervous System Diseases 2020 5(4):58-64

Background and objectives: Emerging evidences indicate an invariable relationship between gut dysbiosis and neurobehavioral symptoms of autism spectrum disorder. In India, Ayurveda is widely accepted among the complementary and alternative medicine. This study aimed to assess the efficacy of an Ayurveda gut therapy protocol in autism spectrum disorder. Subjects and methods: In this randomized controlled trial, 60 children with autism spectrum disorder admitted to Vaidyaratnam P S Varier Ayurveda College, India will be randomly assigned to intervention and control groups. The intervention group will undergo Ayurveda gut therapy protocol for 30 days and interdisciplinary interventions for 2 months, whereas the control group will undergo only interdisciplinary interventions for 2 months. A final assessment will be done on the 60 th day. Patient recruitment began in July 2018. The primary and secondary outcome measure will be completed in January 2021 and the study will be completed in September 2022. The study was approved by the Institutional Ethical Committee of Vaidyaratnam P S Varier Ayurveda College, India (Proceedings No: IEC/CI/24/17) on May 4, 2017. Protocol version: 1.0. Outcome measures: The expected primary outcome is to assess the quality and quantity of the gut microbes through 16s rRNA sequencing. The secondary outcome expected is the changes in the neurobehavioral symptoms assessed through the Childhood Autism Rating Scale and also changes in the gastrointestinal symptoms assessed through Ayurveda Gut Health Assessment Questionnaire. Discussion: The current protocol discusses the relationship between Autism and gut dysbiosis and its management through Ayurveda, and provides evidence for the rationality of using Ayurveda gut therapy as an alternative therapy for autism spectrum disorder in clinical practice. Trial registration: The study was registered with Clinical Trial Registry of India (registration No. CTRI/2018/05/014017, registered on May 21, 2018).



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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
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Academic Medicine

Humanizing the Morbidity and Mortality Conference
Morbidity and mortality conferences (MMCs) are a long-held legacy institution in academic medicine that enable medical providers and hospital administrators to learn from systemic and individual errors, thereby leading to improved medical care. Originally this forum had 1 major role—education. The MMC evolved and a second key role was added: quality improvement. In the wake of the 2020 COVID-19 pandemic, a second evolution—one that will humanize the MMC—is required. The pandemic emphasizes the need to use MMCs not only as a place to discuss errors but also as a place for medical providers to reflect on lives lost. The authors' review of the literature regarding MMCs indicates that most studies focus on enabling MMCs to become a forum for quality improvement, while none have emphasized the need to humanize MMCs to decrease medical provider burnout and improve patient satisfaction. Permitting clinicians to be human on the job requires restructuring the MMC to provide a space for reflection and, ultimately, defining a new purpose and charge for the MMC. The authors have 3 main recommendations. First, principles of humanism such as compassion, empathy, and respect, in particular, should be incorporated into traditional MMCs. Second, shorter gatherings devoted to giving clinicians the opportunity to focus on their humanity could be arranged. Third, an MMC focused entirely on the human aspects of medical care could be periodically arranged to provide an outlet for storytelling, artistic expression, and reflection. Humanizing the MMC—a core symposium in clinical medicine worldwide—could be the first step in revitalizing the spirit at the heart of medicine, one dedicated to health and healing. This spirit, which has been eroding as the field of medicine becomes increasingly corporate in structure and mission, is as essential during peaceful times in health care as during a pandemic. Funding/Support: None reported. Other disclosures: None reported. Ethical approval: Reported as not applicable. Disclaimers: The views expressed in this article are those of the authors and do not necessarily reflect the position or policy of the Department of Veterans Affairs or the United States government. Correspondence should be addressed to Haider J. Warraich, 4B-132, 1400 VFW Parkway, Boston VA Healthcare System, Boston, MA 02132; telephone: (617) 323-7700; email: hwarraich@partners.org; Twitter: @haiderwarraich. Written work prepared by employees of the Federal Government as part of their official duties is, under the U.S. Copyright Act, a "work of the United States Government" for which copyright protection under Title 17 of the United States Code is not available. As such, copyright does not extend to the contributions of employees of the Federal Government. © 2020 by the Association of American Medical Colleges

Perceptual Facilitators for and Barriers to Career Progression: A Qualitative Study With Female Early Stage Investigators in Health Sciences
Purpose: Despite efforts to increase the representation of women in the national scientific workforce, results still lag. While women's representation in health-related sciences has increased substantially, women remain underrepresented in senior leadership roles. This study was conducted to elucidate influences at the individual, interpersonal, organizational, and societal levels that present as barriers to and facilitators for advancement in research careers for women, with the goal of promoting and retaining a more diverse leadership. Method: The authors conducted individual, 1-hour, in-depth, semistructured interviews with 15 female early stage investigators pursuing careers in health sciences research at a large minority-serving institution in Florida in 2018. Interview guides were designed by using a social ecological framework in order to understand the influence of multilevel systems. Employing a qualitative approach, drawing from a phenomenological orientation, 2 researchers independently coded transcripts and synthesized codes into broad themes. Results: Barriers and facilitators were reported at all ecological levels explored. Illustrative quotations reflect the unequal distribution of familial responsibilities that compete with career advancement, family members' lack of understanding of the demands of a research career, the importance of female mentors, perceived differences in the roles and expectations of female and male faculty at institutions, and normative upheld values that influence early career progression. Conclusions: Achieving pervasive and sustained changes that move toward gender equity in research requires solutions that address multilevel, explicit and implicit influences on women's advancement in science. Suggestions include shifting familial and institutional norms, creating support systems for women with female mentors, and enforcing consistent policies regarding the roles and expectations of faculty. Findings shed light on the influence of gender on career progression by providing context for the experiences of women and underscore the importance of addressing pervasive societal and structural systems that maintain inequities hindering women's progress in the scientific workforce. Supplemental digital content for this article is available at http://links.lww.com/ACADMED/B57. Acknowledgments: The authors would like to thank the participants who took part in the study, without whom this work would not be possible. Funding/Support: This research was supported in part by the National Institute on Minority Health and Health Disparities of the National Institutes of Health under award NIMHD U54MD012393, Florida International University Research Center in Minority Institutions. Other disclosures: None reported. Ethical approval: This study was approved by the institutional review board for the study site located in South Florida, as recorded in application IRB-18-0268, reference number 106877. Disclaimer: The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Previous presentations: This work was presented at the RCMI (Research Centers in Minority Institutions) Translational Science Conference, Capacity Building and Investigator Development track; December 2019; Bethesda, Maryland. Correspondence should be addressed to Sofia B. Fernandez, 11200 SW 8th Street, AHC4-328, Miami, FL 33199; telephone: (305) 348-0365; fax: (305) 348-5801; email: sofernan@fiu.edu. © 2020 by the Association of American Medical Colleges

Hispanic Identity and Its Inclusion in the Race Discrimination Discourse in the United States
As protests against racism occur all over the United States and medical institutions face calls to incorporate antiracism and health equity curricula into professional training and patient care, the antiracism discourse has largely occurred through a Black/African American and White lens. Hispanics, an umbrella category created by the U.S. government to include all people of Spanish-speaking descent, are the largest minority group in the country. Hispanics are considered an ethnic rather than a racial group, although some Hispanics self-identify their race in terms of their ethnicity and/or country of origin while other Hispanics self-identify with any of the 5 racial categories used by the U.S. government (White, Black or African American, American Indian or Alaska Native, Asian, or Native Hawaiian or Other Pacific Islander). Expanding the antiracism discourse in medicine to include Hispanic perspectives and the diversity of histories and health outcomes among Hispanic groups is crucial to addressing inequities and disparities in health and medical training. A lack of inclusion of Hispanics has contributed to a growing shortage of Hispanic physicians and medical school faculty in the United States as well as discrimination against Hispanic physicians, trainees, and patients. To reverse this negative trend and advance a health care equity and antiracist agenda, the authors offer steps that medical schools, academic medical centers, and medical accreditation and licensing bodies must take to increase the representation of Hispanics and foster their engagement in this evolving antiracism discourse. Acknowledgments: The authors wish to acknowledge the work of the Latino Medical Student Association, the National Hispanic Medical Association, and all organizations representing Hispanics in medicine, both past and present. Funding/Support: None reported. Other disclosures: None reported. Ethical approval: Reported as not applicable. Correspondence should be addressed to Cristina R. Fernández, Department of Pediatrics, Columbia University Irving Medical Center, 630 West 168th Street, PH-17 Room 201-I, New York, NY 10032; telephone: (212) 342-1758; email: crf2101@cumc.columbia.edu; Twitter: @DrCFernandez. © 2020 by the Association of American Medical Colleges

Everyday Heroism: Maintaining Organizational Cultures of Wellness and Inclusive Excellence Amid Simultaneous Pandemics
Health care professionals and the institutions in which they work are being stretched to their limits amidst the current COVID-19 pandemic. At the same time, a second longstanding pandemic has been brought to the fore: the entrenched system of racial injustice and oppression. The first pandemic is new and to date substantial resources have been allocated to urgently addressing its mitigation; the second has a long history with inconsistent attention and resources but has recently been spotlighted more intensely than at any time in the nation's recent past. The authors of this article contend that these 2 simultaneous pandemics have brought forth the need for institutions in the United States to make a renewed commitment to respect, wellness, diversity, and inclusion. While investment and leadership in these domains have always been essential, these have largely been viewed as a "nice-to-have" option. The events of much of 2020 (most notably) have illustrated that committing to and investing in policies, programs, centers, and leadership to drive change in these domains are essential and a "need-to-have" measure. The authors outline the necessity of investing in the promotion of cultures of inclusive excellence at both individual and organizational levels to coordinate a united response to the simultaneous pandemics. It is in the interests of health care systems to consider the wellness of the workforce to overcome the longer term economic, systemic, and social trauma that will likely occur for years to come at both the individual and institutional levels. Maintaining or augmenting investment is necessary despite the economic challenges the nation faces. Now is the time to cultivate resilience and wellness through a renewed commitment to cultures of respect, diversity, and inclusion. This commitment is urgently needed to support and sustain the health care workforce and maintain outstanding health care systems for future generations. Funding/Support: None reported. Other disclosures: None reported. Ethical approval: Reported as not applicable. Correspondence should be addressed to Magali Fassiotto, PhD, Stanford University School of Medicine, Stanford, CA, 94305; telephone: (650) 723-6078; email: magali.fassiotto@stanford.edu; twitter: @StanfordMedOFDD. © 2020 by the Association of American Medical Colleges

Novel Prescriptions From Medical Schools for Physician–Scientist Training and Engagement in the Twenty-First Century
Physicians engaged in biomedical research are well positioned to directly focus the discovery process on human biology. However, the relative proportion of investigators engaged in both caring for patients and conducting research is decreasing. To address the dwindling numbers of physician–scientists nationally, the Burroughs Wellcome Fund (BWF) created the Physician-Scientist Institutional Awards Program by dedicating 25 million dollars to new initiatives at 10 degree-granting, accredited medical schools in North America, awarded on the basis of institutions' proposals. The perceived barriers to physician–scientist training, program initiatives, and commitment to training a diverse group of future researchers were articulated in each application. In all, the BWF review committee considered 136 distinct proposals from 83 medical schools, representing 54% of all accredited medical schools in North America. Barriers identified by more than one-third of the applicant institutions included the absence of both mentors and role models, student indebtedness, institutional cultures that valued clinical care delivery above the discovery process, limited prior relevant research experience, and structural barriers that limited scheduling flexibility during training. Awards were granted to institutions with programs designed to be sustainable and overcome critical, prospectively identified barriers to training and retention of physician–scientists. Potential solutions from the 10 funded programs were focused on different stages of the training experience. Though a determination about the relative success of each of the initiatives will take many years, careful consideration of the barriers identified and more general application of specific program component may be beneficial in increasing the numbers of physicians actively involved in biomedical research. Funding/Support: None reported. Other disclosures: None reported. Ethical approval: Reported as not applicable. Correspondence should be addressed to John E. Burris, Burroughs Wellcome Fund, 21 T.W. Alexander Drive, P.O. Box 13901, Research Triangle Park, NC 27709-3901; email: jburris@bwfund.org. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. © 2020 by the Association of American Medical Colleges

Recognizing Cross-Institutional Fiscal and Administrative Barriers and Facilitators to Conducting Community-Engaged Clinical and Translational Research
Purpose: This qualitative study examined fiscal and administrative (i.e., pre- and post-award grants process) barriers and facilitators to community-engaged research among stakeholders across 4 Clinical and Translational Science Awards (CTSA) institutions. Method: A purposive sample of 24 key informants from 3 stakeholder groups—community partners, academic researchers, and research administrators—from the CTSA institutions at the University of North Carolina at Chapel Hill, Medical University of South Carolina, Vanderbilt University Medical Center, and Yale University participated. Semistructured interviews were conducted in March–July 2018, including questions about perceived challenges and best practices in fiscal and administrative processes in community-engaged research. Transcribed interviews were independently reviewed and analyzed using the Rapid Assessment Process to facilitate key theme and quote identification. Results: Community partners were predominantly Black, academic researchers and research administrators were predominantly White, and women made up two-thirds of the overall sample. Five key themes were identified: level of partnership equity, partnership collaboration and communication, institutional policies and procedures, level of familiarity with varying fiscal and administrative processes, and financial management expectations. No stakeholders reported best practices for the institutional policies and procedures theme. Cross-cutting challenges included communication gaps between stakeholder groups, lack of or limits in supporting community partners' fiscal capacity, and lack of collective awareness of each stakeholder group's processes, procedures, and needs. Cross-cutting best practices centered on shared decision-making and early and timely communication between all stakeholder groups in both pre- and post-award processes. Conclusions: Findings highlight the importance of equitable processes, triangulated communication, transparency, and recognizing and respecting different financial management cultures within community-engaged research. This work can be a springboard used by CTSA institutions to build on available resources that facilitate co-learning and discussions between community partners, academic researchers, and research administrators on fiscal readiness and administrative processes for improved community-engaged research partnerships. Supplemental digital content for this article is available at http://links.lww.com/ACADMED/B55. Acknowledgments: Adina Black and Elisa D. Quarles served as reviewers in the Rapid Assessment Process. Adina Black provided administrative support in developing this manuscript. Jennifer Teixeira, director of research administration in the Office of Sponsored Research at University of North Carolina at Chapel Hill, contributed to the conceptualization of this project. The authors thank the stakeholders—community partners, academic researchers, and research administrators—for participating in this study. Funding/Support: This work was supported, in part, by the Clinical and Translational Science Awards Program, funded by the National Center for Advancing Translational Sciences of the National Institutes of Health: grants #UL1TR002489 (University of North Carolina at Chapel Hill), #UL1TR001450 (Medical University of South Carolina), #UL1TR002243 (Vanderbilt University Medical Center), and #UL1TR001863 (Yale University). Other disclosures: None reported. Ethical approval: The University of North Carolina at Chapel Hill Institutional Review Board approved this study on September 8, 2017 (IRB#15-0849). Disclaimers: The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Correspondence should be addressed to Lori Carter-Edwards, University of North Carolina at Chapel Hill, Campus Box 7064, 160 N. Medical Dr., Chapel Hill, NC 27599; telephone: (919) 966-5305; email: lori_carter-edwards@unc.edu; Twitter: @NCTraCS. This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2020 by the Association of American Medical Colleges

Effect of Continuing Professional Development on Health Professionals' Performance and Patient Outcomes: A Scoping Review of Knowledge Syntheses
Purpose: Continuing professional development (CPD) programs, which aim to enhance health professionals' practice and improve patient outcomes, are offered to practitioners across the spectrum of health professions through both formal and informal learning activities. Various knowledge syntheses (or reviews) have attempted to summarize the CPD literature; however, these have primarily focused on continuing medical education or formal learning activities. Through this scoping review, the authors seek to answer the question, What is the current landscape of knowledge syntheses focused on the impact of CPD on health professionals' performance defined as behavior change and/or patient outcomes? Method: In September 2019, the authors searched PubMed, Embase, CINAHL, Scopus, ERIC, and PsycINFO for knowledge syntheses published between 2008 and 2019 that focused on independently practicing health professionals and reported outcomes at Kirkpatrick's levels 3 and/or 4. Result: Of the 7,157 citations retrieved from databases, 63 satisfied the inclusion criteria. Of these 63 syntheses, 38 (60%) included multicomponent approaches, and 27 (43%) incorporated eLearning interventions – either stand-alone or in combination with other interventions. While a majority of syntheses (n = 42 [67%]) reported outcomes affecting health care practitioners' behavior change and/or patient outcomes, most of the findings reported at Kirkpatrick level 4 were not statistically significant. Ten of the syntheses (16%) mentioned the cost of interventions though this was not their primary focus. Conclusions: Across health professions CPD is an umbrella term incorporating formal and informal approaches in a multi-component approach. eLearning is increasing in popularity but remains an emerging technology. Several of the knowledge syntheses highlighted concerns regarding both the financial and human costs of CPD offerings, and such costs are being increasingly addressed in the CPD literature. Supplemental digital content for this article is available at http://links.lww.com/ACADMED/B56. Acknowledgments: The authors would like to thank Rhonda Allard, a medical librarian at Uniformed Services University of the Health Sciences, for helping design, refine, and conduct the searches for this study. Funding/Support: None reported. Other disclosures: None reported. Ethical approval: Reported as not applicable. Disclaimer: The opinions and assertions expressed herein are those of the author(s) and do not necessarily reflect the official policy or position of the Uniformed Services University of the Health Sciences or the Department of Defense or the Henry M. Jackson Foundation for Military Medicine. Correspondence should be addressed to Anita Samuel, Uniformed Services University of the Health Sciences, Department of Medicine, Graduate Programs in Health Professions Education, 4301 Jones Bridge Road, Bethesda, MD 20814; telephone: (301) 295-9539; email: anita.samuel.ctr@usuhs.edu. Written work prepared by employees of the Federal Government as part of their official duties is, under the U.S. Copyright Act, a "work of the United States Government" for which copyright protection under Title 17 of the United States Code is not available. As such, copyright does not extend to the contributions of employees of the Federal Government. © 2020 by the Association of American Medical Colleges

Personalized Graduate Medical Education and the Global Surgeon: Training for Resource-Limited Settings
Problem: The World Health Organization and the World Bank have identified improvement in access to surgical care as an urgent global health challenge and a cost-effective investment in public health. However, trainees in standard U.S. general surgery programs do not have adequate exposure to the procedures, technical skills, and foundational knowledge essential for providing surgical care in resource-limited settings. Approach: The Michael E. DeBakey Department of Surgery at Baylor College of Medicine (BCM) created a 7-year global surgery track within its general surgery residency in 2014. Individualized rotations equip residents with the necessary skills, knowledge, and experience to operate in regions with low surgeon density and develop sustainable surgical infrastructures. BCM provides a formal, integrated global surgery curriculum—including 2 years dedicated to global surgery—with surgical specialty rotations in domestic and international settings. Residents tailor their individual experience to the needs of their future clinical practice, region of interest, and surgical specialty. Outcomes: There have been 4 major outcomes of the BCM global surgery track: (1) increased exposure for trainees to a broad range of surgeries critical in resource-limited settings, (2) meaningful international partnerships, (3) contributions to global surgery scholarship, and (4) establishment of sustainable global surgery activities. Next Steps: To better facilitate access to safe, timely, and affordable surgical care worldwide, global surgeons should pursue expertise in topics not currently included in U.S. general surgical curricula, such as setting-specific technical skills, capacity building, and organizational collaboration. Future evaluations of the BCM global surgery track will assess the effect of individualized education on trainees' professional identities, clinical practices, academic pursuits, global surgery leadership preparedness, and comfort with technical skills not encompassed in general surgery programs. Increasing availability of quality global surgery training programs would provide a critical next step towards contributing to the delivery of safe surgical care worldwide. Acknowledgements: The authors wish to thank Miriam King, MEd, Scott LeMaire, MD, Chad Wilson, MD, MPH, C. Anne Morrison, MD, MPH, Walter Johnson, MD, MPH, MBA, Neema Kaseje, MD, MPH, Nader Masserweh, MD, MPH, Josephine Koller, BBA, Sydney Webster, MEd, Jaye Chambers, Allyson Bremer, Woods McCormack, MA, Bip Nandi, MBBChir, Heather Vasser, MD, Kathryn Gunter, MD, Michael Coburn, MD, Michael Belfort, MBBCH, DA (SA), MD, PhD, Jeffrey Wilkinson, MD, Rachel Pope, MD, MPH, Kelli Barbour, MD, Candy Wilburn, Etan Weinstock, MD, Peter Hotez, MD, PhD, John Dawson, MD, Christopher Perkins, MD, MS, Adam Gibson, JD, Taylor Napier, MA, Hisashi Nikaidoh, MD, Lynn Nikaidoh, Craig Brown, Sue Smith, JD, and John Collier, MDiv, MA. Funding/Support: Funding and support for the creation of the global surgery residency track were received from the Baylor College of Medicine Michael E. DeBakey Department of Surgery, Hitoshi Nikaidoh Memorial Endowment, George A. Robinson IV Foundation, Craig and Galen T. Brown Foundation, CHRISTUS Foundation for HealthCare, Caring Friends in Deed, and the Bridget L. Harrison, MD International Education Support Fund. Other disclosures: None reported. Ethical approval: Reported as not applicable. Correspondence should be addressed to Rachel W. Davis, Baylor College of Medicine, 1 Baylor Plaza, MS390, Houston, TX, 77030; telephone: (713) 798-6078; email: rachelwdavis@bcm.edu; Twitter: @RachelWDavis. © 2020 by the Association of American Medical Colleges

Discharge Communication: A Multi-Institutional Survey of Internal Medicine Residents' Education and Practices
Purpose: To characterize residents' practices around hospital discharge communication and their exposure to transitions-of-care instruction in graduate medical education (GME). Method: In spring 2019, internal medicine residents at 7 academic medical centers completed a cross-sectional survey reporting the types of transitions-of-care instruction they experienced during their GME training and the frequency with which they performed 6 key discharge communication practices. The authors calculated a mean discharge communication score for each resident and, using multiple logistic regression, they analyzed the relationship between exposure to types of educational experiences and the discharge communication practices that residents reported to perform frequently (> 60% of time). The authors also used content analysis to explore factors that motivated residents to change their discharge practices. Results: The response rate was 63.5% (613/966). Resident discharge communication practices varied. Notably, only 17.0% (n = 104) reported routinely asking patients to "teach-back" or explain their understanding of the discharge plans. The odds of frequently performing key discharge communication practices were greater if residents received instruction based on observation of and feedback regarding their communication with patients at discharge (adjusted odds ratio [OR] 1.73; 95% confidence interval [CI], 1.07-2.81), or if they received explicit on-rounds teaching (adjusted OR 1.46; 95% CI, 1.04-2.230). In open-ended comments, residents reported that experiencing adverse patient events at some point in the post-discharge continuum was a major impetus for practice change. Conclusions: This study exposes gaps in hospital discharge communication with patients, highlights the benefits of workplace-based instruction on discharge communication skills, and reveals the influence of adverse events as a source of hidden curricula. The results suggest that developing faculty to incorporate transitions-of-care instruction in their rounds teaching and integrating experiences across the post-discharge continuum into residents' education may foster physicians-in-training who are champions of effective transitions of care within the fragmented healthcare system. Supplemental digital content for this article is available at http://links.lww.com/ACADMED/B54. Acknowledgements: The authors would like to thank Shreya Singhal and Megan Sutter, PhD, for statistical assistance, as well as Amy Ou, MD, Masha Slavin, MD, Bilal Alqam, MD, Marina Baskharoun, MD, Nick Gowen, MD, Paul Williams, MD, and Derek Hupp, MD, for help distributing the survey. Funding/Support: Dr. Shreya P. Trivedi's time was supported by Health Resources and Services Administration-T32 grant (T32HP22238). Other disclosures: None reported. Ethical approval: Ethical approval was received from each participating institution. Previous presentations: The findings of this study were presented as a virtual oral presentation for the Lipkin Finalist Award for the Society of General Internal Medicine On-Demand 2020 National Conference. Data: The data for this study were not collected from outside sources. Correspondences should be addressed to Shreya Trivedi, Department of Medicine, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, Massachusetts 02215; telephone: (215) 527-9238; email: strived1@bidmc.harvard.edu; Twitter: @ShreyaTrivediMD. © 2020 by the Association of American Medical Colleges

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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
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Haematology

Prognostic impact of Annexin A1 expression in acute myeloid leukemia
Mohmoud Gaber, Ali M Kasem, Mohamed Azzazi, Mohamed Tarif, Emad A Yusuf

The Egyptian Journal of Haematology 2020 45(2):57-67

Objective The aim was to assess expression of Annexin A1 (ANXA1) on malignant myeloid blast cells and its correlation with clinical outcome, overall survival (OS), and other prognostic factors among adult Egyptian patients with acute myeloid leukemia (AML). Patients and methods A total of 60 patients with de novo AML were treated and followed up in Ain Shams University Hospitals, Hematology Unit and compared with 20 age-matched and sex-matched normal healthy controls. Expression of ANXA1 was detected by flow cytometry in bone marrow samples. Results Patients with AML had significant higher mean ANXA1 expression than mean ANXA1 expression in control at D0. The mean ANXA1 level in the favorable cytogenetics group was significantly higher than the mean ANXA1 level in the unfavorable cytogenetics group. ANXA1 was positive in 76.7% patients with AML and negative in 23.3% patients with AML. There was a significant difference between ANXA1-positive group and ANXA1-negative group of patients with AML regarding different outcomes. In ANXA1-positive group, 52.2% of patients with AML achieved complete remission (CR), whereas in ANXA1-negative group, 14.3% of patients with AML achieved CR. Highest mean ANXA1 expression was in the group of patients who had CR. High ANXA1 expression was associated with longer OS. Conclusion ANXA1 is significantly expressed in Egyptian patients with de novo AML, and its expression associated with favorable prognostic effect on clinical outcome and longer OS.


Assessment of serum interleukin-15 in adult acute leukemia patients (Egyptian sample)
Mohamed M Moussa, Mahmoud Sheeba, Emad Abdel Mohsen, Mohamed Elshazly, Nour E Hussein

The Egyptian Journal of Haematology 2020 45(2):68-76

Background Interleukin 15 (IL-15), proinflammatory cytokine, regulates immune system functions and controls hematopoietic cell differentiation. It promotes leukemia development through enhancing survival, proliferation, and differentiation of leukemic precursors. Aim The aim was to assess the expression of IL-15 level in adult Egyptian acute leukemia patients, its correlation with disease-free survival, overall survival and relapse rate, and its possible correlation with other prognostic parameters. Patients and methods Serum IL-15 was measured using ELISA in 30 newly diagnosed acute lymphoblastic leukemia (ALL) patients, 30 newly diagnosed acute myeloid leukemia (AML) patients, and 30 healthy controls recruited from Ain Shams University Hospital from 2017 to 2018. Results In ALL patients IL-15 was higher in patients compared with the control. IL-15 was higher in patients with non-high-risk cytogenetics compared with those with high-risk cytogenetics. Patients with high IL-15 who achieved first complete response (CR) were less than those with low/average IL-15. Patients with high IL-15 who achieved minimal residual disease (MRD) negativity were less than those with low/average IL-15. Patients with high IL-15 had shorter survival compared with those with low/average IL-15 level. Optimal cutoff value for IL-15 in predicting patient survival in ALL patients was 200&#8201;ng/l. In AML patients: IL-15 was higher in patients compared with the control. IL-15 was higher in patients with high-risk features compared with those without. Patients with high IL-15 achieved first CR less than those with average IL-15. Patients with high IL-15 achieved MRD negativity less than those with average IL-15. Patients with high IL-15 had shorter survival compared with those with low/average IL-15. Optimal cutoff value for IL-15 in predicting patient survival in AML patients was 190&#8201;ng/l. IL-15 has negative correlation with death date in AML patients. Conclusion IL-15 is a useful poor prognostic marker in newly diagnosed acute leukemia patients&#8217; also it can be used as a predictor for CR, MRD, and survival.


Lymphocyte DNA damage in children with iron-deficiency anemia: a case–control study
Aneseya P Varghese, Smriti Sinha, Seema P Sindgikar, Rathika D Shenoy, Vijaya Shenoy

The Egyptian Journal of Haematology 2020 45(2):77-80

Context Iron-deficiency anemia (IDA) is a widespread yet one of the most neglected micronutrient deficiency disorder worldwide. Aims The objective was to evaluate lymphocyte DNA damage in children with anemia. Materials and methods This was a prospective case&#8211;control study. A total of 80 infants and children aged six months to twelve years were included. Fifty had IDA, whereas 30 were controls. DNA damage scoring using alkaline comet assay was done in all children. Percentage and mean and/or SEM were calculated. Comparison between cases and controls was done using Student&#8217;s t test. Analysis of variance test was used to compare the groups within the cases and controls. Pearson correlation coefficient was performed to relate the hemoglobin levels with the DNA damage. Statistical software was used for analysis. Results DNA damage scoring was significant in all the parameters in the iron-deficiency group with respect to the tail length and percentages of DNA in tail and olive moment, with a P value of 0.006, 0.002, and 0.038, respectively. A statistically significant negative correlation was found between hemoglobin levels and percentage of DNA in tail (r&#61;&#8722;0.280; P&#61;0.012) as well as olive moment (r&#61;&#8722;0.240; P&#61;0.032) Conclusion IDA was associated significantly with lymphocyte DNA damage. A significant negative correlation between hemoglobin levels and percentage of DNA and olive moment was also elucidated. The authors conclude that early intervention is needed in even mild cases of IDA.


Prognostic value of platelet glycoprotein Ibα (Kozak) gene polymorphism in patients with coronary heart disease
Mohamed Sabry El-Ghonemy, Solafa El Sharawy, Mahmoud M AbdoYouseif, Shaimaa El-Ashwah, Menna T-Allah Fayez, Ahmed EL-Sebaie

The Egyptian Journal of Haematology 2020 45(2):81-86

Background Coronary heart disease (CHD) is one of the leading causes of death worldwide for both men and women. It is caused by the disproportion between myocardial oxygen demand and its supply. Platelets play an important role in thrombosis and hemostasis by forming a plug at the exposed subendothelium preventing blood loss; if this process is uncontrolled it results in thrombotic events causing life-threatening disease such as myocardial infarction (MI) or ischemic stroke. Glycoprotein (GP) Ib-IX-V is a platelet membrane receptor complex containing four polypeptides, GPIb&#945;, GPIbB, GPIX, and GPV, which plays a key role in mediating platelet activity and thrombosis. This study aimed to evaluate the role of platelet GPIb&#945; (Kozak) gene polymorphism as a risk factor of CHD. Patients and methods This study was conducted on 120 newly diagnosed patients of CHD admitted to the Internal Medicine Hospital [46 patients with unstable angina (UA) and 74 patients with MI]. Thirty apparently healthy individuals served as a control group. The EDTA blood samples collected from patients and the control group were subjected to DNA extraction, followed by PCR amplification for the Kozak gene. Results This study showed that by taking rs2243093 TT as the reference genotype and T as the reference allele. TC, CC, TC+CC genotypes, and C allele showed a lower frequency in cases when compared with the control group, with protective effect against CHD susceptibility (P&#60;0.05). On the other hand, no association was found in GP1b&#945; genotypes and alleles between UA and MI subgroups (P&#62;0.05). Conclusion Individuals carrying the C allele of (rs2243093) had protective effect against CHD including UA, MI. The rs2243093 had no association with the risk of MI in those having UA. Wild genotype (TT) was associated with higher creatine kinase-MB, while genotypes containing the risk allele (C) had lower creatine kinase-MB in CHD patients.


Sociodemographic and clinical determinants of folate deficiency among sickle cell anemia patients in Kano, North Western Nigeria
Ibrahim Abdulqadir, Aisha A Galadanci, Mujtaba I Mashi, Sagir A Gumel, Aisha K Gwarzo

The Egyptian Journal of Haematology 2020 45(2):87-91

Context Folate deficiency is common among sickle cell anemia (SCA) patients and could be influenced by factors such as clinical and social conditions. Sociodemographic and clinical determinants of folate deficiency have not been previously investigated among SCA patients in this environment. Aims The aim was to determine the sociodemographic and clinical factors associated with folate deficiency in SCA patients accessing care at Aminu Kano Teaching Hospital, Kano. Settings and design This was a cross-sectional study involving 110 SCA participants at steady state. Patients and methods Sociodemographic and clinical data of the participants were collated, while their folate status was determined using Roche Elecsys 2010. Statistical analysis Data were analyzed with SPSS version 21.0 and level of significance was set at P less than or equal to 0.05. Results The mean age of the participants was 14.9&#177;7.1 years and the majority of them were men (58, 52.7%), students (96, 87.3%), and jaundiced (66, 60.0%). Age, sex, occupation of the participants, and mother&#8217;s level of education were significantly associated with folate deficiency (P&#60;0.05). Female gender [odds ratio (95% confidence interval) for red blood cells 2.6 (1.2&#8211;5.7) and serum 2.8 (1.3&#8211;6.1)] and mothers with less than secondary education [odds ratio (95% confidence interval) for red blood cells 5.3 (1.0&#8211;27.6) and serum 4.2 (1.0&#8211;18.1)] were independent predictors of folate deficiency. Conclusion Sociodemographic and clinical characteristics are important determinants of folate deficiency in SCA. We therefore recommend periodic assessment of folate status as part of the standard care to SCA patients to prevent complications of folate deficiency.


A potential immunological diagnostic marker for acute myeloid leukemia: the expression of the immune checkpoint B and T lymphocyte attenuator
Sara M Radwan, Nooran S Elleboudy, Nermeen A Nabih, Amal A El-kholy, Amany M Kamal

The Egyptian Journal of Haematology 2020 45(2):92-96

Introduction Provided the physiologic tumor suppressive role a healthy immune system has, diagnosis of cancer is sometimes also regarded as a diagnosis of an immune dysfunction. Immunoediting refers to the change of immune system response from tumor protection to tumor promotion, favoring the growth of poorly immunogenic clones that have the ability to evade the immune response. Among the mechanisms by which the tumor cells bypass the immune system is immune checkpoints. Which are immune inhibitory pathways responsible for self-tolerance and limiting self-tissue damage by controlling the magnitude and duration of the immune reaction. Objective Studying the expression of the novel immune checkpoint BTLA in patients with AML and evaluate its clinicopathological and diagnostic significance. Patients and methods We investigated the expression of BTLA gene in 60 AML cases and 15 healthy controls using RT-PCR. Results The current study revealed that significant up regulation of BTLA mRNA expression in AML patients as in comparison with the control group (P&#61;0.024). Discussion These results provide a basis for the perception that BTLA upregulation is involved in inhibition of antitumor immunity and that high BTLA expression level can be made use of as a diagnostic marker in patients with AML.


Longitudinal assessment of the impact of tuberculosis infection and treatment on monocyte–lymphocyte ratio, neutrophil–lymphocyte ratio, and other white blood cell parameters
Chizoba O Okeke, Grace I Amilo, Martin O Ifeanyichukwu, Ejeatuluchukwu O Obi

The Egyptian Journal of Haematology 2020 45(2):97-104

Context Tuberculosis (TB) is a major infectious disease usually marked by alterations in white blood cell (WBC) parameters which are known to play a major role in the normal body response to infections. Aim The aim was to assess the impact of TB infection and treatment on monocyte-to-lymphocyte ratio (MLR), neutrophil-to-lymphocyte ratio (NLR), and other WBC parameters in TB individuals before and in the course of therapy. Settings and design This was a longitudinal follow-up study that included 60 TB-infected individuals, age 18 and 80 years. The TB individuals were recruited at Mile-Four Hospital Abakaliki before initiation of therapy and followed up at 2 months and 6 months into treatment. Materials and methods TB diagnosis was done using both Ziehl&#8211;Neelsen acid fast bacilli test and GeneXpert Mycobacterium tuberculosis/rifampicin assay. Whole blood collected in dipotassium ethylenediamine tetraacetic acid was used for the measurement of total white blood cell count and differential white cell count (neutrophil, lymphocyte, monocyte, eosinophil) and packed cell volume. The MLR and NLR were calculated. Statistical Package for the Social Sciences (SPSS), version 22 was used for statistical analysis. Results The findings showed that total white cell count, neutrophil count, lymphocyte count, eosinophil count, and monocyte count (&#215;109/l) were all significantly decreased after 2-month treatment compared with the pretreatment values (P&#60;0.05) and all except monocyte was significantly increased after 6-month treatment (P&#60;0.05). There was a significant decrease in NLR and MLR after 2 months of treatment that was maintained after 6 months of treatment. Moreover, the packed cell volume (l/l) increased significantly after 2 months of treatment and decreased significantly after 6 months of treatment. Conclusion TB is associated with significant changes in NLR, MLR, and other WBC parameters which might be possible markers to explore as a prognostic index in TB disease.


β-catenin mutations in acute myeloid leukemia
Buket A Gunes, Tulin Ozkan, Aynur K Gurel, Yalda Hekmatshoar, Meral Beksac, Asuman Sunguroglu

The Egyptian Journal of Haematology 2020 45(2):105-110

Background Acute myeloid leukemia (AML) is a hematopoietic stem cell disorder characterized by uncontrolled proliferation and impaired differentiation of normal hematopoietic stem or progenitor cells. Various pathways like RAF/MEK/ERK, PI3K/AKT, receptor tyrosine kinases, members of RAS family, and Wnt/&#946;-catenin are disrupted in AML. Stabilization of &#946;-catenin, the key point of activated Wnt/&#946;-catenin pathway, has been shown in AML in various studies. One of the mechanisms that may lead to the &#946;-catenin stabilization is the mutations in exon 3 at its N-terminal domain, where &#946;-catenin is phosphorylated particularly during the degradation process, and these mutations have been investigated in chronic myeloid leukemia and many other cancer types. Aim &#946;-Catenin gene exon 3 mutations were analyzed in this study with the aim of determining the relationship between mutations and molecular biology of AML. Patients and methods In this study, we examined &#946;-catenin gene mutations in AML cell line U937 and 31 untreated patients with AML by using the DNA sequence analysis for the first time in a Turkish population. Results No &#946;-catenin gene exon 3 mutations were detected in patients with AML and the cell line. Conclusion &#946;-catenin mutations have been detected in various types of cancer; however, the authors did not find any mutation in this gene, which may be responsible for the activation of Wnt signaling in AML. Further research is required to understand the mechanisms apart from mutations that may induce &#946;-catenin stabilization in AML.


Hyperhomocysteinemia is associated with deep vein thrombosis in Nigerian patients
Sunday P Ogundeji, Taiwo R Kotila, Foluke A Fasola

The Egyptian Journal of Haematology 2020 45(2):111-114

Background Hyperhomocysteinemia is a modifiable risk factor associated with deep venous thrombosis (DVT). Low serum concentrations of vitamin B12 and folate are modifiable causes of hyperhomocysteinemia. This study was carried out to determine the prevalence of hyperhomocysteinemia in Nigerian patients with DVT and also to assess the association of homocysteine with vitamin B12 and folate. Patients and methods A case&#8211;control study was carried out in which serum homocysteine, vitamin B12, and folate levels were measured in 45 Doppler ultrasound confirmed cases of DVT and 43 controls. Hyperhomocysteinemia was defined by serum levels more than 15.0&#8201;&#956;mol/l, low serum folate less than 3&#8201;&#956;g/l, and low serum vitamin B12 less than 160&#8201;ng/l. Results The prevalence of hyperhomocysteinemia in DVT patients was 20 (44.4%) compared with four (9.35%) in the control (P&#60;0.001). The mean serum folate and vitamin B12 levels of DVT patients were 8.7&#177;4.8 and 725&#177;475, while that of the controls were 9.3&#177;3.8&#8201;&#956;g/l and 821&#177;393&#8201;ng/l (P&#61;0.51 and 0.36), respectively. There was no correlation between hyperhomocysteinemia and low vitamin B12 and folate levels. Conclusion Hyperhomocysteinemia may be a risk factor for DVT in the Nigerian population but may not be responsible for thrombosis in all cases. There was however no significant correlation between serum level of homocysteine and serum levels of vitamin B12 and folate in DVT patients. Therefore, larger sized sample studies should be carried out to investigate the relationship between hyperhomocysteinemia and related vitamins as well as genetic causes of hyperhomocysteinemia in DVT patients in our environment.


Burkitt lymphoma presenting with nasopharyngeal mass and generalized lymphadenopathy in a HIV-positive patient
Manasi Mundada, Faiq Ahmed, Rachna Khera, Sudha Murthy, Pavan Kumar Boyella

The Egyptian Journal of Haematology 2020 45(2):115-117

Burkitt lymphoma is an aggressive B-cell neoplasm arising from the germinal center B cells. Clinically, it exists in three forms: endemic, sporadic, and immunodeficiency associated. Immunodeficiency-associated Burkitt lymphoma is usually extranodal and occurs with a CD4 count more than 200&#8201;&#956;l. We report a retrospective study of a 47-year-old male patient, seropositive for HIV infection, presenting with nasopharyngeal mass and generalized lymphadenopathy. His CD4 count was 192 cells/&#956;l, and lactate dehydrogenase was high. Diagnosis was established by biopsy, immunohistochemistry, and fluorescence in-situ hybridization. He was treated with chemotherapy. The disease progressed to involve central nervous system despite treatment. He received palliative radiotherapy and was stable at 11-month follow-up. This report highlights the uncommon clinical presentation, heavy disease burden, and progression of the disease in a HIV seropositive patient.



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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
Telephone consultation 11855 int 1193,

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