SIMULTANEOUS DETERMINATION OF FLURBIPROFEN AND PANTOPRAZOLE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

2016-11-08T01-11-23Z
Source: Indo American Journal of Pharmaceutical Research
Varaprasad Adepu*, K.E.V Nagoji, V.Girijasastry.
RP-HPLC method is developed for determination of pantoprazole and flurbiprofen in binary combination. Pantoprazole and flurbiprofen were detected at 270 nm using a BDS Hypersil C8 (250 X 4.6 mm, 5 μm) column and mixture of 0.02M phosphate buffer (pH 3.0) and acetonitrile 30:70 v/v as mobile phase.The flowrate was 1ml/min and effluent was detected at 270nm. Response was linear over the range of 10-100 mcg/ml for pantoprazole and of 5-50 mcg/ml for flurbiprofen. The retention time of flubiprofen was 2.407 min and pantoprazole was 5.441 min.The developed method efficiently separated the analytical peaks from mixture. The method was validated according to ICH guidelines with respect to specificity, linearity, accuracy, precision, LOD and LOQ. The method developed can be applied successfully for determination of pantoprazole and flurbiprofen in dosage form.


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